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polyclonal sheep anti human cr2  (R&D Systems)


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    R&D Systems polyclonal sheep anti human cr2
    PBMCs from healthy donors were incubated with or without 30 µg/ml MBP in medium containing normal autologous serum (30% v/v), or in pure medium. The resulting deposition of C3 and C1q on B cells was measured by flow cytometry after 5 min incubation (N = 3). Representative histogram plots show A) C3-deposition, and B) C1q-deposition on B cells. C) The binding of MBP was assessed using biotinylated MBP as probe and subsequent staining with streptavidin-PE. Blockade of CR1 or <t>CR2</t> was achieved by pre-incubation of PBMCs with mAb3D9 and <t>polyclonal</t> sheep anti-human CR2 respectively. Monoclonal anti-glycophorin (GP)-A was used as negative control. D) Mean fluorescence intensity (MFI) values of 5–6 experiments are shown; background values (of samples with no MBP added) have been subtracted. Bars and error bars represent means and SEM. **p<0.01, ***p<0.001.
    Polyclonal Sheep Anti Human Cr2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal sheep anti human cr2/product/R&D Systems
    Average 90 stars, based on 2 article reviews
    polyclonal sheep anti human cr2 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Uptake and Presentation of Myelin Basic Protein by Normal Human B Cells"

    Article Title: Uptake and Presentation of Myelin Basic Protein by Normal Human B Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0113388

    PBMCs from healthy donors were incubated with or without 30 µg/ml MBP in medium containing normal autologous serum (30% v/v), or in pure medium. The resulting deposition of C3 and C1q on B cells was measured by flow cytometry after 5 min incubation (N = 3). Representative histogram plots show A) C3-deposition, and B) C1q-deposition on B cells. C) The binding of MBP was assessed using biotinylated MBP as probe and subsequent staining with streptavidin-PE. Blockade of CR1 or CR2 was achieved by pre-incubation of PBMCs with mAb3D9 and polyclonal sheep anti-human CR2 respectively. Monoclonal anti-glycophorin (GP)-A was used as negative control. D) Mean fluorescence intensity (MFI) values of 5–6 experiments are shown; background values (of samples with no MBP added) have been subtracted. Bars and error bars represent means and SEM. **p<0.01, ***p<0.001.
    Figure Legend Snippet: PBMCs from healthy donors were incubated with or without 30 µg/ml MBP in medium containing normal autologous serum (30% v/v), or in pure medium. The resulting deposition of C3 and C1q on B cells was measured by flow cytometry after 5 min incubation (N = 3). Representative histogram plots show A) C3-deposition, and B) C1q-deposition on B cells. C) The binding of MBP was assessed using biotinylated MBP as probe and subsequent staining with streptavidin-PE. Blockade of CR1 or CR2 was achieved by pre-incubation of PBMCs with mAb3D9 and polyclonal sheep anti-human CR2 respectively. Monoclonal anti-glycophorin (GP)-A was used as negative control. D) Mean fluorescence intensity (MFI) values of 5–6 experiments are shown; background values (of samples with no MBP added) have been subtracted. Bars and error bars represent means and SEM. **p<0.01, ***p<0.001.

    Techniques Used: Incubation, Flow Cytometry, Binding Assay, Staining, Negative Control, Fluorescence

    PBMCs from healthy HLA-DR15+ donors were incubated for 18 h with MBP in media containing normal serum. Cells were stained with FITC anti-CD19 and biotinylated MK16, followed by streptavidin-PE. (A) The binding of MK16 at different serum concentrations is shown as mean fluorescence (MFI) values normalised to that of 10% serum, (N = 4). B) Before addition of serum (30% v/v), different concentrations of the complement inhibitory compound sodium polyanethole sulphonate (SPS) were added. MFI values are shown, normalised to samples without SPS, (N = 6). (C) The PBMCs were pre-incubated with the anti-CR1 mAb3D9 or polyclonal sheep anti-human CR2, or both, before addition of serum (30% v/v) and MBP. Anti-glycophorin (GP)-A was used as negative control. Data are shown as means±SEM, (N = 4–6). **p<0.01.
    Figure Legend Snippet: PBMCs from healthy HLA-DR15+ donors were incubated for 18 h with MBP in media containing normal serum. Cells were stained with FITC anti-CD19 and biotinylated MK16, followed by streptavidin-PE. (A) The binding of MK16 at different serum concentrations is shown as mean fluorescence (MFI) values normalised to that of 10% serum, (N = 4). B) Before addition of serum (30% v/v), different concentrations of the complement inhibitory compound sodium polyanethole sulphonate (SPS) were added. MFI values are shown, normalised to samples without SPS, (N = 6). (C) The PBMCs were pre-incubated with the anti-CR1 mAb3D9 or polyclonal sheep anti-human CR2, or both, before addition of serum (30% v/v) and MBP. Anti-glycophorin (GP)-A was used as negative control. Data are shown as means±SEM, (N = 4–6). **p<0.01.

    Techniques Used: Incubation, Staining, Binding Assay, Fluorescence, Negative Control



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    polyclonal sheep anti human cr2  (R&D Systems)
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    R&D Systems polyclonal sheep anti human cr2
    PBMCs from healthy donors were incubated with or without 30 µg/ml MBP in medium containing normal autologous serum (30% v/v), or in pure medium. The resulting deposition of C3 and C1q on B cells was measured by flow cytometry after 5 min incubation (N = 3). Representative histogram plots show A) C3-deposition, and B) C1q-deposition on B cells. C) The binding of MBP was assessed using biotinylated MBP as probe and subsequent staining with streptavidin-PE. Blockade of CR1 or <t>CR2</t> was achieved by pre-incubation of PBMCs with mAb3D9 and <t>polyclonal</t> sheep anti-human CR2 respectively. Monoclonal anti-glycophorin (GP)-A was used as negative control. D) Mean fluorescence intensity (MFI) values of 5–6 experiments are shown; background values (of samples with no MBP added) have been subtracted. Bars and error bars represent means and SEM. **p<0.01, ***p<0.001.
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    https://www.bioz.com/result/polyclonal sheep anti human cr2/product/R&D Systems
    Average 90 stars, based on 1 article reviews
    polyclonal sheep anti human cr2 - by Bioz Stars, 2026-03
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    sheep polyclonal antibodies anti human complement receptor 2 cr2  (R&D Systems)
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    PBMCs from healthy donors were incubated with or without 30 µg/ml MBP in medium containing normal autologous serum (30% v/v), or in pure medium. The resulting deposition of C3 and C1q on B cells was measured by flow cytometry after 5 min incubation (N = 3). Representative histogram plots show A) C3-deposition, and B) C1q-deposition on B cells. C) The binding of MBP was assessed using biotinylated MBP as probe and subsequent staining with streptavidin-PE. Blockade of CR1 or <t>CR2</t> was achieved by pre-incubation of PBMCs with mAb3D9 and <t>polyclonal</t> sheep anti-human CR2 respectively. Monoclonal anti-glycophorin (GP)-A was used as negative control. D) Mean fluorescence intensity (MFI) values of 5–6 experiments are shown; background values (of samples with no MBP added) have been subtracted. Bars and error bars represent means and SEM. **p<0.01, ***p<0.001.
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    Image Search Results


    PBMCs from healthy donors were incubated with or without 30 µg/ml MBP in medium containing normal autologous serum (30% v/v), or in pure medium. The resulting deposition of C3 and C1q on B cells was measured by flow cytometry after 5 min incubation (N = 3). Representative histogram plots show A) C3-deposition, and B) C1q-deposition on B cells. C) The binding of MBP was assessed using biotinylated MBP as probe and subsequent staining with streptavidin-PE. Blockade of CR1 or CR2 was achieved by pre-incubation of PBMCs with mAb3D9 and polyclonal sheep anti-human CR2 respectively. Monoclonal anti-glycophorin (GP)-A was used as negative control. D) Mean fluorescence intensity (MFI) values of 5–6 experiments are shown; background values (of samples with no MBP added) have been subtracted. Bars and error bars represent means and SEM. **p<0.01, ***p<0.001.

    Journal: PLoS ONE

    Article Title: Uptake and Presentation of Myelin Basic Protein by Normal Human B Cells

    doi: 10.1371/journal.pone.0113388

    Figure Lengend Snippet: PBMCs from healthy donors were incubated with or without 30 µg/ml MBP in medium containing normal autologous serum (30% v/v), or in pure medium. The resulting deposition of C3 and C1q on B cells was measured by flow cytometry after 5 min incubation (N = 3). Representative histogram plots show A) C3-deposition, and B) C1q-deposition on B cells. C) The binding of MBP was assessed using biotinylated MBP as probe and subsequent staining with streptavidin-PE. Blockade of CR1 or CR2 was achieved by pre-incubation of PBMCs with mAb3D9 and polyclonal sheep anti-human CR2 respectively. Monoclonal anti-glycophorin (GP)-A was used as negative control. D) Mean fluorescence intensity (MFI) values of 5–6 experiments are shown; background values (of samples with no MBP added) have been subtracted. Bars and error bars represent means and SEM. **p<0.01, ***p<0.001.

    Article Snippet: Murine anti-human CR1 IgG1 antibody (mAb3D9) was kindly donated by Dr John O'Shea (Frederick Cancer Research and Development Center, Frederick, MD, USA), and polyclonal sheep anti-human CR2 was purchased from R&D Systems (Minneapolis, MN, USA).

    Techniques: Incubation, Flow Cytometry, Binding Assay, Staining, Negative Control, Fluorescence

    PBMCs from healthy HLA-DR15+ donors were incubated for 18 h with MBP in media containing normal serum. Cells were stained with FITC anti-CD19 and biotinylated MK16, followed by streptavidin-PE. (A) The binding of MK16 at different serum concentrations is shown as mean fluorescence (MFI) values normalised to that of 10% serum, (N = 4). B) Before addition of serum (30% v/v), different concentrations of the complement inhibitory compound sodium polyanethole sulphonate (SPS) were added. MFI values are shown, normalised to samples without SPS, (N = 6). (C) The PBMCs were pre-incubated with the anti-CR1 mAb3D9 or polyclonal sheep anti-human CR2, or both, before addition of serum (30% v/v) and MBP. Anti-glycophorin (GP)-A was used as negative control. Data are shown as means±SEM, (N = 4–6). **p<0.01.

    Journal: PLoS ONE

    Article Title: Uptake and Presentation of Myelin Basic Protein by Normal Human B Cells

    doi: 10.1371/journal.pone.0113388

    Figure Lengend Snippet: PBMCs from healthy HLA-DR15+ donors were incubated for 18 h with MBP in media containing normal serum. Cells were stained with FITC anti-CD19 and biotinylated MK16, followed by streptavidin-PE. (A) The binding of MK16 at different serum concentrations is shown as mean fluorescence (MFI) values normalised to that of 10% serum, (N = 4). B) Before addition of serum (30% v/v), different concentrations of the complement inhibitory compound sodium polyanethole sulphonate (SPS) were added. MFI values are shown, normalised to samples without SPS, (N = 6). (C) The PBMCs were pre-incubated with the anti-CR1 mAb3D9 or polyclonal sheep anti-human CR2, or both, before addition of serum (30% v/v) and MBP. Anti-glycophorin (GP)-A was used as negative control. Data are shown as means±SEM, (N = 4–6). **p<0.01.

    Article Snippet: Murine anti-human CR1 IgG1 antibody (mAb3D9) was kindly donated by Dr John O'Shea (Frederick Cancer Research and Development Center, Frederick, MD, USA), and polyclonal sheep anti-human CR2 was purchased from R&D Systems (Minneapolis, MN, USA).

    Techniques: Incubation, Staining, Binding Assay, Fluorescence, Negative Control